《植物生理学报》 2018, 54(5): 886-894

菊花基因组DNA甲基化水平对根系硝态氮吸收的影响

于媛媛, 郭芸珲, 温立柱, 孙翠慧, 樊红梅, 孙宪芝, 王文莉, 孙霞, 郑成淑^*

山东农业大学园艺科学与工程学院/山东省中日韩菊花国际合作研究中心, 山东泰安271018

通信作者：郑成淑；E-mail: zcs@sdau.edu.cn

摘　要：

以菊花的扦插生根苗为试材, 在缺氮和适氮水培条件下, 分别使用甲基化抑制剂(5-azaC)和甲基供体(SAM)处理菊花根系, 测定处理后基因组DNA甲基化水平变化、根系构型相关参数、根系硝态氮含量以及NO₃^-转运蛋白基因CmNRT1.1、CmNRT2.1、CmNAR2.1的相对表达量。结果表明, 在适氮条件下, 5-azaC处理的菊花根系DNA甲基化水平降低, 根系总直径、总表面积及总体积增大; 根系NO₃^-含量与对照相比显著增加, 根系NO₃^-转运蛋白基因CmNRT1.1、CmNRT2.1、CmNAR2.1相对表达量也有所升高。据此推测, 降低菊花根系基因组DNA甲基化水平可以提高根系NO₃^-转运相关基因的表达, 进而促进根系对NO₃^-的吸收。

关键词：菊花; 根系; DNA甲基化; 硝态氮; 5-azaC; SAM; MSAP

收稿：2017-11-20   修定：2018-04-13

资助：国家科技支撑计划项目(2011BAD10B07)和国家自然科学基金面上项目(31670663)。

Effects of DNA methylation level on the nitrate uptake of roots in Chrysanthemum morifolium

YU Yuan-Yuan, GUO Yun-Hui, WEN Li-Zhu, SUN Cui-Hui, FAN Hong-Mei, SUN Xian-Zhi, WANG Wen-Li, SUN Xia, ZHENG Cheng-Shu^*

College of Horticulture Science and Engineering, Shandong Agricultural University, Chrysanthemum Research Center of China, Japan and Korea in Shandong Province, Taian, Shandong 271018, China

Corresponding author: ZHENG Cheng-Shu; E-mail: zcs@sdau.edu.cn

Abstract:

Using the rooted cuttings of chrysanthemum (Chrysanthemum morifolium) as test materials, the chrysanthemum roots system was treated with methylation inhibitor (5-azaC) and methyl donor (SAM) under the condition of nitorgen deficiency and suitable nitrogen hydroponics. The changes of DNA methylation level, root system configuration parameters, the contents of NO₃^- and the relative expression levels of NO₃^- transporter gene CmNRT1.1, CmNRT2.1 and CmNAR2.1 were measured. The results showed that the DNA methylation level with 5-azaC treatment was reduced; the total root diameter, total surface area and total volume of root system increased. In addition, the contents of NO₃^- was significantly increased and the relative expression levels of CmNRT1.1, CmNR2.1 and CmNAR2.1 in root system were also increased. It is conjectured that the reduction of DNA methylation level in the genome of chrysanthemum could increase the expression of NO₃^- transport related genes, and then promote the absorption of NO₃^- in the root system.

Key words: Chrysanthemum morifolium; root; DNA methylation; nitrate nitrogen; 5-azaC; SAM; MSAP